This video lecture talks about Ion exchange chromatography in Hindi, Ion Exchange chromatography, cation exchange chromatography, anion exchange chromatography, ion exchange chromatography practicle, lab procedure, PI of Protein, Zwitter ion, Ph of buffer solution in ion exchange chromatography. Ion exchange chromatogram This lecture explains about the instrumentation, principle of Ion exchange Chromatography. Ion chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. However, ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein.The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged. The molecule is positively charged because the pH for chromatography is less than the pI. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. Anion-exchange chromatography is when the stationary phase is positively charged and negatively charged molecules (meaning that pH for chromatography is greater than the pI) are loaded to be attracted to it.It is often used in protein purification, water analysis, and quality control. The water-soluble and charged molecules such as proteins, amino acids, and peptides bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase.The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column—a cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations. Cation exchange chromatography is used when the desired molecules to separate are cations and anion exchange chromatography is used to separate anions. Article source: wikipedia Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel ! Subscribe: https://www.youtube.com/channel/UCU91ilky-nR0VwKYFmJTsUA?sub_confirmation=1 HPLC Chromatography: https://youtu.be/9P_SIiMheZk UV Visible Spectroscopy: https://youtu.be/1mrUHrZ-0zk #BaaYo #Chromatography
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This comment about the video lecture explains about ion exchange chromatography principle. It also explains the step-by-step process of ion exchange chromatography. That includes the the cation exchange chromatography and anion exchange chromatography. Ion exchange chromatography involves using positive and negative in charge ions to separate charged molecules from a mixture. In the positive this is a problem was in video. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching
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Ion Exchange Chromatography, Cationic Exchanger, Anionic Exchanger, Stationary Phase in Ion Exchange Chromatography, Mobile Phase in Ion Exchange Chromatography, Solvent System in Ion Exchange Chromatography, CM- Cellulose, DEAE, TEAE
Views: 6998 Dr. Puspendra Classes
Education, Medicine, Health, Healthy lifestyle, Physics, Chemistry, Maths, Nihilist
Views: 149 Nihilist
Subject: Biophysics Paper:Techniques used in molecular biophysics I
Views: 116 Vidya-mitra
All our Principles and Methodology handbooks are available here: https://www.gelifesciences.com/handbooks Ion exchange chromatography is one of the most frequently used techniques for purification of biomolecules and separates the molecules according to differences in their net surface charge. This video describes the principles of the technique.
Views: 418897 GE Healthcare Life Sciences
http://technologyinscience.blogspot.com/2013/07/an-insight-on-ion-exchange.html The principle of Ion Exchange chromatography separation is the reversible interaction of charged species with the ion exchange matrix. Ion Exchange Chromatography is of Two types: Cation Exchange and Anion Exchange.
Views: 156119 Bio-Resource
http://www.gurug.net Unit-3 Application of Adsorption, Role of Adsobents in Catalysis,Ion Exchange Adsorption,Pollution Abatement - Chemistry
Views: 1175 Kutty School
Subscribe today and give the gift of knowledge to yourself or a friend adsorption ion exchange resins ADSORPTION ION EXCHANGE RESINS. BIOCHEMISTRY DR AMINA TARIQ. ADSORPTION. Adsorption, the binding of molecules or particles to a surface. Absorption , the filling of pores in a solid. Slideshow 2979193 by tanuja show1 : Adsorption ion exchange resins show2 : Adsorption show3 : Adsorption ion exchange resins show4 : Adsorption ion exchange resins show5 : Adsorption ion exchange resins show6 : Adsorption ion exchange resins show7 : Adsorption ion exchange resins show8 : Adsorption ion exchange resins show9 : Adsorption ion exchange resins show10 : Adsorption ion exchange resins show11 : Adsorption ion exchange resins show12 : Adsorption ion exchange resins show13 : Adsorption ion exchange resins show14 : Adsorption ion exchange resins show15 : Adsorption ion exchange resins show16 : Adsorption ion exchange resins show17 : Adsorption ion exchange resins show18 : Adsorption ion exchange resins show19 : Adsorption ion exchange resins show20 : Adsorption ion exchange resins show21 : Adsorption ion exchange resins show22 : Adsorption ion exchange resins show23 : Adsorption ion exchange resins show24 : Adsorption ion exchange resins show25 : Adsorption ion exchange resins show26 : Adsorption ion exchange resins show27 : Adsorption ion exchange resins show28 : Adsorption ion exchange resins show29 : Adsorption ion exchange resins show30 : Adsorption ion exchange resins show31 : Applications
Views: 125 Magalyn Melgarejo
The principle of Ion exchange chromatography is based on the attraction of oppositely charged particle. Many of the biological compounds such as protein, amino acid have charges. These charges can be used in the separation of the compounds by using ion exchanger. The Exchangers are Anionic exchanger which are positively charged and Cationic exchanger which are negatively charged.
Views: 595 Microbiology Tube
For more information, visit http://www.bio-rad.com/yt/26/ngc. Shawn Anderson presents an introduction to ion exchange chromatography using Bio-Rad's chromatography instruments, columns, and media to demonstrate the technique. Shawn covers the basic principles of ion exchange chromatography and touches on applications for the separation of proteins and other biomolecules using a variety of anion and cation exchange media. Bio-Rad's Successful Chromatography Webinar series provides a great introduction to the different chromatography methods available for research applications. Most medium pressure chromatography columns work with Bio-Rad's NGC preparative chromatography system. The NGC is designed to rapidly automate the purification of biomolecules. The flexible, modular, and economical design makes the NGC systems the instruments of choice for method development and scale-up. NGC systems are available in six preplumbed, factory-tested configurations with two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps to meet specific application needs. Any system can be configured for operation at either high or low flow rate by simply changing the pump head on the system pump module. With the NGC system, a single hardware platform can be modified as needed to suit changing applications and scales. . http://www.bio-rad.com/evportal/destination/product?catID=MFCV7415&WT.mc_id=yt-pfd-ww-ngc-20130701-zytDmnoioJo We Are Bio-Rad. Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges. Connect with Bio-Rad Online: Website: http://www.bio-rad.com/ LinkedIn: https://www.linkedin.com/company/1613226/ Facebook: https://www.facebook.com/biorad/ Twitter: https://twitter.com/BioRadLifeSci Instagram: @BioRadLabs Snapchat: @BioRadLabs
Views: 36603 Bio-Rad Laboratories
We go over the three main types of chromatography to separate your protein of interest. We got a fancy new drawing tablet so all our drawings will be digital now instead of on a whiteboard :). Artist: Kyle Simpson Narrator: Sophie Tsai
Views: 199 BehindTheBench
http://www.pakwatercare.org Deionizers -- Demineraliser Pakwater Care Services is a leading provider of deionization solutions. Our water deionizers are rugged, pre-engineered, pre-assembled, standardized units that minimize expensive installation and start-up costs. We have designed our Deionization systems to maximize the efficiency and repeatability of the unit during the service and regeneration modes The Process of Deionization or Ion-Exchange In the context of water purification, ion-exchange is a rapid and reversible process in which impurity ions present in the water are replaced by ions released by an ion-exchange resin. The impurity ions are taken up by the resin, which must be periodically regenerated to restore it to the original ionic form. (An ion is an atom or group of atoms with an electric charge. Positively-charged ions are called cations and are usually metals; negatively-charged ions are called anions and are usually non-metals). The following ions are widely found in Raw Waters: Cations Anions Calcium (Ca2+) Chloride (Cl-) Magnesium (Mg2+) Bicarbonate (HCO3-) Sodium (Na+) Nitrate (NO3-) Potassium (K+) Carbonate (CO32-) Iron (Fe2+) Sulfate (SO42-) Ion Exchange Resins There are two basic types of resin -- cation-exchange and anion-exchange resins. Cation exchange resins will release Hydrogen (H+) ions or other positively charged ions in exchange for impurity cations present in the water. Anion exchange resins will release hydroxyl (OH-) ions or other negatively charged ions in exchange for impurity anions present in the water. The application of ion-exchange to Water Treatment and Purification There are three ways in which ion-exchange technology can be used in water treatment and purification: first, cation-exchange resins alone can be employed to soften water by base exchange; secondly, anion-exchange resins alone can be used for organic scavenging or nitrate removal; and thirdly, combinations of cation-exchange and anion-exchange resins can be used to remove virtually all the ionic impurities present in the feedwater, a process known as deionization. Water deionizers purification process results in water of exceptionally high quality. Deionization For many laboratory and industrial applications, high-purity water which is essentially free from ionic contaminants is required. Water of this quality can be produced by deionization.The two most common types of deionization are: * Dual-Bed Deionization * Mixed-Bed Deionization Dual-Bed Deionization The two-bed deionizer consists of two vessels -- one containing a cation-exchange resin in the hydrogen (H+) form and the other containing an anion resin in the hydroxyl (OH-) form. Water flows through the cation column, whereupon all the cations are exchanged for hydrogen ions.To keep the water electrically balanced, for every monovalent cation, e.g. Na+, one hydrogen ion is exchanged and for every divalent cation, e.g. Ca2+, or Mg2+, two hydrogen ions are exchanged. The same principle applies when considering anion-exchange. The decationised water then flows through the anion column. This time, all the negatively charged ions are exchanged for hydroxide ions which then combine with the hydrogen ions to form water (H2O). PAKWATER CARE SERVICES http://www.pakwatercare.org Follow us http://www.facebook.com/pakwater http://www.twitter.com/pakwater http://pk.linkedin.com/in/pakwater
Views: 16683 pakwatercarepk
www.gelifesciences.com/ProteinResearch Watch this 50-minute webinar and learn how to: • Optimize the resolution/purity of your target protein • Maximize protein recovery • Reduce hands-on time by optimizing your system’s configuration • Select the right ion exchange chromatography columns and resins to boost your results and efficiency
Views: 414 GE Healthcare Life Sciences
Engineered for medium-sized deionization (DI) applications, these dependable units feature the same precision electronics and performance characteristics as their large-scale counterparts, all in a smaller, top mounted package. Applications cover the full spectrum of industrial, commercial, medical and agricultural users. As a rugged, multipurpose system, dual bed deionizers are all-around systems that serve a wide array of applications. Application: Deionized Water for Data Center Features: • High Pressure Pump • Filmtec XLE-2540 Membranes • FRP Pressure Vessels • Pre-Filter Housing with 5 Micron Cartridges • Reject and Product Flow Meters • Pre and Post Pressure Gauges • Low Pressure Switch • Inlet SS 316 Solenoid Valve • High Quality DI Resin Media • Effluent Resistivity 14-18 MΩ-cm • Factory Tested for Reliability and Designed to Work Seamlessly • Easy Installation • High Capacity • Long Service Intervals and Easy Maintenance • Longer Membrane Life For more information, please visit our website: https://www.pureaqua.com/commercial-reverse-osmosis-ro-systems/ https://www.pureaqua.com/dual-bed-deionizer/ #MadeByPureAqua #MadeByPureAqua
Views: 78 Pure Aqua, Inc. (USA)
Subject:Analytical Chemistry/Instrumentation Paper: Chromatographic techniques
Views: 109 Vidya-mitra
Weitere Informationen über Amazon-Deutschland: http://bit.ly/291Nf2p
Views: 0 Moshe Hyman
To know more about our products, please visit our website www.i-2-m.com or contact us at [email protected] We are proud to introduce our ion exchange filter, the PROTECT+ion Omniflow. PROTECT+ion Omniflow protects equipment from excessive ionic charges. This is accomplished by using high capacity resins to provide low conductivity in an easy to service, ion exchange filter. The PROTECT+ion Omniflow performs exceptionally well in most liquid systems and is specifically designed for bypass flow applications. Our ion exchange filter comes in four sizes to cover a wide range of applications and meet all customers’ requirements. PROTECT+ion Omniflow is the ideal addition to a hydrogen fuel cell cooling circuit or closed loop cooling system. However, there is a need for deionization in many other industries such as the medical and aerospace fields. The new PROTECT+ion Omniflow is the best ion exchange filter on the market for fuel cells and high-value applications.
Views: 270 i-2-m
-- Created using PowToon -- Free sign up at http://www.powtoon.com/youtube/ -- Create animated videos and animated presentations for free. PowToon is a free tool that allows you to develop cool animated clips and animated presentations for your website, office meeting, sales pitch, nonprofit fundraiser, product launch, video resume, or anything else you could use an animated explainer video. PowToon's animation templates help you create animated presentations and animated explainer videos from scratch. Anyone can produce awesome animations quickly with PowToon, without the cost or hassle other professional animation services require.
Views: 67 MOOC 260
Ion exchange chromatography can be done using either an anion or a cation exchanger. When we have to separate cations from a mixture of anions, and neutral molecules, then we should use cation exchange chromatography. More details of ion-exchange can be viewed from the website, at the following link: http://www.biokaryon.com/lecture-3-ion-exchange-chromatography/
Views: 1711 BioKaryon
Envirochemie's ion exchanger plants detoxify industrial wastewater and ensure the production of water at the required quality levels. Ion exchangers are used in the treatment of industrial process water and wastewater. Our ion exchanger plants are proven to be successful in a wide range of applications, for the treatment of water circulation systems, removal of residual solids, recovery of precious metals and detoxification of wastewater. The technology is used in industries such as the metal-processing, chemical and pharmaceutical industries, electroplating, or manufacturing and processing. EnviroChemie is pleased to undertake the regeneration of ion exchange resins for you. Sorted by industries of origin, we regenerate each type of resin separately.
Views: 133 EnviroChemie GmbH
Oligosaccharide profiling of biopharmaceutical and other glycoprotein products remains challenging for scientists who report that regardless of the technique used, more than one technique is often required for complete oligosaccharide identification. Recent collaborative studies have noted that while MALDITOF MS was faster, High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD), provided significantly better precision, allowing identification of structural isomers and simultaneous analysis of neutral and sialylated oligosaccharides.
Views: 2281 GENNews
The use of an organic scavenger is an inexpensive and regenerable solution to remove both natural and synthetic organic matter from water streams. The right resin selection depends on the type of organic compound in the water. Learn more: Visit Answer Center: http://dowwater.custhelp.com/app/answers/detail/a_id/121/kw/121. More on Dow Ion Exchange Resins: http://www.dowwaterandprocess.com/en/products/ion_exchange_resins, and organic scavengers: http://www.dowwaterandprocess.com/en/industries-and-applications/industrial_water/other_industrial_water/organic_scavengers. Tap the Tap the Answer Center knowledge pool with your questions: http://dowwater.custhelp.com/app/home
Views: 1301 The Dow Chemical Company
QY Research Reports introduced market research and analysis report on Global And China Ion Exchange Resin Industry 2013 2018 Report at http://www.qyresearchreports.com/report/global-and-china-ion-exchange-resin-industry-2013-2018-report.htm This report also includes more info about basic overview of the industry including definitions, applications and industry china structure industry overview; international market analysis China domestic market and Global market analysis Macroeconomic environment and economic situation analysis and influence Ion-exchange Resin industry policy and plan Ion-exchange Resin Product specification manufacturing process product cost structure etc. For more details visit at : http://www.qyresearchreports.com/ Table of Contents Chapter One Ion-exchange Resin Overview 1 1.1 Ion-exchange Resin Definition 1 1.2 Ion-exchange Resin Classification and Application 2 1.3 Ion-exchange Resin Industry Chain Structure 5 1.4 Ion-exchange Resin Industry Overview 5 1.5 Development History 6 1.6 Competition Pattern 8 1.7 International and Domestic Markets Comparison 9 Chapter Two Ion-exchange Resin China Market Analysis 10 2.1 2013 Global and China Key Manufacturers Ion-exchange Resin product price list 10 2.2 2013 Global and China Key Manufacturers Ion-exchange Resin product profit margin list 11 2.3 2013 Global and China Key Manufacturers Ion-exchange Resin product capacity and capacity share list 12 2.4 2013 Global and China Key Manufacturers Ion-exchange Resin product production and production share list 13 2.5 2013 Global and China Key Manufacturers Ion-exchange Resin product production value and production value share list 14 Chapter Three Ion-exchange Resin Production Technology Overview 15 3.1 2013 Global and China Key Manufacturers Ion-exchange Resin product advantages comparison 15 3.2 2013 Global and China Key Manufacturers Ion-exchange Resin product production line overview 19 3.3 2013 Global and China Key Manufacturers Ion-exchange Resin product origin 24 3.4 2013 Global and China Key Manufacturers Ion-exchange Resin product R&D analysis 25 3.5 2013 Global and China Key Manufacturers Ion-exchange Resin product equipment list 27 3.6 2013 Global and China Key Manufacturers Ion-exchange Resin product raw material analysis 29 Chapter Four Ion-exchange Resin Policy and Industry News 31 4.1 Policy analysis 31 4.2 Industry news 33 4.3 Industry development trend analysis 34 Chapter Five Ion-exchange Resin Manufacturing Process and Cost Structure 36 5.1 Ion-exchange Resin product specification 36 5.2 Ion-exchange Resin Manufacturing Process analysis 41 5.3 Ion-exchange Resin Cost Structure analysis 46 Chapter Six 2009-2014 Global and China Ion-exchange Resin Productions Supply Sales Demand Market Status and Forecast Analysis 47 6.1 2009-2014 Ion-exchange Resin capacity production statistics 47 6.2 2009-2014 Ion-exchange Resin capacity production market share list 55 6.3 2009-2014 Ion-exchange Resin production capacity utilization 58 6.4 2009-2014 Ion-exchange Resin price profit margin list 59 6.5 2009-2014 Ion-exchange Resin production value overview 61 6.6 2009-2014 Ion-exchange Resin product types statistics 62 6.7 2009-2014 Ion-exchange Resin product application statistics 65 6.8 2009-2014 Ion-exchange Resin product region statistics 66 6.9 2009-2014 Ion-exchange Resin demand overview 67 6.10 2009-2014 Ion-exchange Resin supply demand shortage 67 6.11 2009-2014 Ion-exchange Resin import export consumption 67 6.12 2009-2014 Ion-exchange Resin average cost price production value profit margin 68 See also China Ion-Exchange Resin Market Analysis, Ion-Exchange Resin Market Trend Research, 2013 Ion-Exchange Resin Growth Trends
Views: 310 QY Research Reports