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Brain Aneurysm Clipping with the Leica M530 OH6 Neurosurgical  Microscope | Vik Udani, MD
 
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Recording of a brain aneurysm clipping by Vik Udani, MD, Division of Neurosurgery, Senta Clinic, San Diego, CA. Patient is a 38-year-old female with a history of hypertension and migraines. She was found to have an 8mm unruptured right middle cerebral artery aneurysm and underwent a right frontal temporal craniotomomy for aneurysm clipping. Dr. Udani on the M530 OH6 surgical microscope: "One of my favorite aspects of the OH6 is the amount of illumination on the surgical field. The light transmission provides sharp, crisp, bright images of fine vascular structures, which greatly enhances my visualization throughout the entire procedure. The improved illumination as well as the integrated camera also provides greater clarity when using the ICG activated filter giving me greater confidence in my surgical decisions." Watch this video and experience for yourself: Bright 400-Watt Xenon light system for concentrated light beam into deep cavities; high resolution and depth of focus with exclusive FusionOptics technology. Find out more about the M530 OH6 premium surgical microscope for neurosurgery: http://www.leica-microsystems.com/products/surgical-microscopes/neurosurgery-spine/details/product/leica-m530-oh6/ Case and video provided courtesy of Vik Udani, MD.
Views: 70681 LeicaMicrosystems
STED Confocal Super-Resolution - Leica TCS SP8 STED 3X
 
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The resolution needed to image subcellular architecture and dynamics in light microscopy is hindered by the diffraction limits as described by Ernst Abbe. Simply stated, structures smaller than 200 nanometers are lost in a blur. However, the field of super-resolution microscopy has produced methods to obtain resolution beyond this limit. Leica Microsystems has pioneered this field and offers the Leica TCS SP8 STED 3X for Stimulated Emission Depletion microscopy. STED instantly produces super-resolution images, compatible with the dynamics of living cells, without the need for post-processing. Learn more at: http://www.leica-microsystems.com/products/confocal-microscopes/leica-tcs-sp8-configurable-confocal/details/product/leica-tcs-sp8-sted-3x/
Views: 9787 LeicaMicrosystems
Leica A60 Stereomicroscopes
 
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The Leica A60 F and Leica A60 S stereomicroscopes make products and components optimally visible, enable subassemblies to be processed easily, and increase productivity. http://www.leica-microsystems.com
Views: 11675 LeicaMicrosystems
IMSI - Intracytoplasmic Morphologically Selected Sperm Injection
 
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Joint Leica Microsystems and Eppendorf Movie on IMSI
Views: 26854 LeicaMicrosystems
White Light Laser - The Leica TCS SP8 X
 
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The Leica White Light Laser produces a continuous spectral output between the wavelengths of 470 and 670 nm. It allows you to select 8 excitation lines from 3 trillion unique combinations for simultaneous imaging of multiple fluorophores. The white light laser source of the Leica TCS SP8 X perfectly matches the wavelength of any fluorophore.
Views: 8063 LeicaMicrosystems
PAULA, how are my cells today? - Standardize the work in your cell culture lab and save time
 
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PAULA, the world’s first Personal Automated Lab Assistant helps you to speed up your daily cell culture tasks and standardize the results to improve your downstream workflow. It is small, enough to stay faithfully by your cells on any lab bench or even directly in an incubator. It has the capability to tell you when your cells are ready for the next step in the workflow. PAULA is fast, smart, and always in contact with you if you want. Automating essential steps allows you to Save time by memorizing your personal settings Take away all the guesswork Stay connected with the lab to monitor your cells at all times With PAULA Cell Imager you will manage your cell cultures instead of just culturing them. For more information visit: https://www.leica-microsystems.com/products/light-microscopes/inverted-microscopes/details/product/paula/ ----------------------------------------------------------------------------------------------------------- (Video transcript) This is PAULA, my Personal Automated Lab Assistant. PAULA helps me manage my cell cultures. You can call it simply a smart cell imager with phase contrast and two fluorescence channels. But PAULA is first of all a personal assistant taking a load of routine tasks off my back. Scan the flask and check the status of each culture. Done in seconds! Even for a larger number of flasks, PAULA makes sure to document fully all the work. The results for cell confluence are now reproducible. From day one, PAULA took away the guesswork that burdened my group for years. Thanks to PAULA, I can access the information for all of my cell cultures at anytime from anywhere and share it. PAULA can even let me know when my cell cultures are ready for the next step. So weekends and holidays are mine again. PAULA can learn new things whenever I need additional cell-based assays. How are my cells today? I can just ask PAULA – my personal automated lab assistant.
Views: 2219 LeicaMicrosystems
Confocal Laser Scanning Microscope Leica TCS SP8
 
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Leica TCS SP8 Confocal Microscope has been designed for confocal microscopy with optimal photon efficiency and high speed. All optical components are matched towards preserving fluorescence photons for image contrast and to improve cell viability in live cell imaging. Backing up this sensitive detection are a high speed scanning system with up to 428 frames per second, large field of view of field number 22 and accelerated Z-stacking by a novel mode for the SuperZ galvanometer called Galvoflow. More: http://www.leica-microsystems.com/products/confocal-microscopes/configurable-confocal-leica-tcs-sp8/details/product/leica-tcs-sp8/
Views: 18495 LeicaMicrosystems
Upright Materials Microscope with LED Illumination Leica DM2700 M
 
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This materials microscope combines high-quality Leica optics with state-of-the-art universal white light LED illumination. It is the ideal inspection tool for all kinds of routine inspection tasks in metallography, earth science, forensic investigation, and materials quality control and research. The Leica DM2700 M shows you how simple and reliable microscopy can be while helping to improve workflow. http://www.leica-microsystems.com/products/light-microscopes/industrial-materials/upright-microscopes/details/product/leica-dm2700-m/
Views: 8735 LeicaMicrosystems
Sectioning of Aluminum with Ultramicrotome Leica EM UC7
 
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Learn more: http://www.leica-microsystems.com/products/em-sample-prep/biological-specimens/room-temperature-techniques/ultramicrotomy/details/product/leica-em-uc7-1/
Views: 4871 LeicaMicrosystems
Save 90% of the time when doing materials analysis
 
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The DM6 M LIBS 2-in-1 microscope comes with integrated chemical analysis 2 systems in 1 for visual & chemical analysis 1 second to a chemical fingerprint 0 sample preparation DONE The new DM6 M LIBS microscope for materials analysis from Leica Microsystems allows you to analyze the 2 main characteristics of a material in 1 work step, i.e., microstructure and composition. The microscope has an integrated laser spectroscopy (LIBS) function and delivers the chemical composition of any microstructure that you see in the field of view within a second. Compared to typical downstream analysis with electron microscopy, this solution saves you up to 90% of the time typically spent on analysis. The smart 2-in-1 system eliminates the need for sample transfer and the time for sample preparation and set up for electron microscopy. All the relevant information to make the right decisions during inspection is available within seconds. With the DM6 M LIBS solution, users can focus in on a material structure of interest, then trigger the LIBS analysis with a single click. The sample remains in air during the entire analysis. The resulting spectra show the fingerprints of the elements present in the area investigated. The chemical composition will be precisely identified. Materials inspection is important for quality control, failure analysis and research and development in multiple industries and fields, like metal alloy, automotive, electronics, and materials science. --------------------------------------------------------------------------- (Video transcript) 2-in-1: Visual and Chemical Inspection Combined Learn how the DM6 M LIBS system delivers full results in only a few moments For material analysis, the visual inspection is just the first step The chemical composition of the microstructure in the image is also important Until now, identifying the chemical composition required a time-consuming downstream analysis The new DM6 M LIBS solution integrates visual and chemical inspection in a single work step - 2 in 1 This means 90% less time for the full analysis of materials compared to SEM/EDS methods Here is your new workflow. Save 90% of the time for a full analysis Visual inspection: Place the sample on the stage of the microscope Search for the area of interest in the field of view Chemical inspection: One click initiates the qualitative chemical inspection with laser spectroscopy Done! Verify the results of the chemical analysis just seconds after the laser shot Find another position on the sample Now let's see how it works Laser pulse strikes the surface Plasma emits characteristic elemental spectra during breakdown Done! The DM6 M LIBS solution from Leica Microsystems For more information visit: https://www.leica-microsystems.com/products/light-microscopes/industrial-materials/upright-microscopes/details/product/libs-module/
Views: 6455 LeicaMicrosystems
Leica EM CPD300 - Automated Critical Point Dryer
 
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Controlled Specimen Drying for Biological and Industrial Applications The Leica EM CPD300 dries specimens such as pollen, tissue, plants, insects, etc. as wells as industrial samples like Micro Electro Mechanical Systems (MEMS) in a fully automated and controlled process.
Views: 4230 LeicaMicrosystems
Sharing Microscope Images on iPad
 
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Leica DMshare provides a live display of what you and the microscope camera can see wireless on one or multiple iPad. http://itunes.apple.com/en/app/leica-dmshare/id526669066?mt=8 http://www.leica-microsystems.com/products/light-microscopes/education/life-science/details/product/leica-dmshare/
Views: 5468 LeicaMicrosystems
Leica EM UC7 Ultramicrotome
 
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The Ultramicrotome Leica EM UC7 provides easy preparation of semi- and ultrathin sections as well as perfect, smooth surfaces of biological and industrial samples for TEM, SEM, AFM and LM examination. http://www.leica-microsystems.com
Views: 25874 LeicaMicrosystems
Optical Innovations and 3D Heads-up Surgery for Ophthalmology
 
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In this video, Dr. Ike Ahmed demonstrates how optical innovations help to improve ophthalmic surgeries. The surgical footage includes the use of an OttoFlex diameter for a pediatric cataract surgery, the demonstration of a wide-angle beam system for a vitreous incarceration and the advantages of a motorized tilting mechanism in angle surgery. In addition, Dr. Ahmed talks about his experience with 3D heads-up surgery, using a stereoscopic high-definition visualization system for cataract, glaucoma, and retinal surgeries. Being at the leading edge of innovative treatments for cataract surgery, Dr. Ahmed was one of the first ophthalmologists to work with 3D heads-up visualization using a Leica M822 ophthalmic microscope. His focus lies on the development of advanced microsurgical techniques in glaucoma surgery and complicated cataract extraction, and he is actively involved in research and medical education at a national and international level. He has received research grants to study glaucoma medications, glaucoma laser and surgical devices/techniques, angle closure glaucoma, anterior segment and retinal/optic nerve imaging in glaucoma, cataract surgical techniques and devices, and intraocular lens designs. Dr. Ahmed has designed innovative glaucoma diamond scalpels for surgery, microsurgical instrumentation, and devices, implants, and techniques for the management of the dislocated cataract, iris reconstruction, and glaucoma implant devices. Dr. Ike Ahmed is Assistant Professor at the University of Toronto, Clinical Assistant Professor at the University of Utah, Fellowship Director, Glaucoma & Anterior Segment Surgery (GAASS) Fellowship at the University of Toronto and Research Fellowship Director at the University of Toronto, Department of Ophthalmology and Vision Sciences. He is a fellowship-trained glaucoma, cataract, and anterior segment surgeon with a practice focus on the surgical management of glaucoma, complex cataract and intraocular lens complications. He is board certified in ophthalmology in Canada and the USA. More information on TrueVision 3D Visualization System for Ophthalmology: http://www.leica-microsystems.com/products/surgical-microscopes/ophthalmology/details/product/truevision-3d-visualization-system-ophthalmology/ More information on the Leica M822 F20/F40: http://www.leica-microsystems.com/products/surgical-microscopes/ophthalmology/details/product/leica-m822-f20/
Views: 8255 LeicaMicrosystems
Leica Application Suite X Microscope Software
 
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Leica Application Suite X (LAS X) is the easy to use software platform for advanced life science research on Leica Microsystems confocal, widefield and super-resolution systems. http://www.leica-microsystems.com/products/microscope-software/software-for-life-science-research/las-x/
Views: 7637 LeicaMicrosystems
Leica DCM8 Optical Surface Metrology System Applications
 
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Digital Microscope System with full HDMI output The Leica DMS300 is a complete digital microscope system that utilizes a HDMI-monitor instead of eyepieces. Leica's high quality 8:1 zoom optics are combined with a 2.5MP camera to provide a full high-definition live image with up to 30fps. The Leica DMS300 produces high-quality, full-color still images as well as Full-HD movies as a standalone system. http://www.leica-microsystems.com/products/stereo-microscopes-macroscopes/macroscopes/details/product/leica-dms300/
Views: 2289 LeicaMicrosystems
FusionOptics technology from Leica Microsystems: Whiteboard video
 
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With FusionOptics technology from Leica Microsystems, you can discover details that were previously invisible using stereo microscopy. The observer receives an image with both outstanding richness of detail and depth of field at the same time. The technology allows you to see more of your sample and provides the necessary working distance so you can comfortably manipulate it under the microscope. For more information, go to https://www.leica-microsystems.com/products/stereo-microscopes-macroscopes/research/details/product/leica-m205-fca/ Video transcript: A typical stereo microscope has an inherent optical limitation that make users choose between a microscope with either a high depth of field or one that maximizes image resolution, forcing the user to make a trade-off. Stereo microscopes with FusionOptics, exclusively from Leica Microsystems, eliminate this trade off, allowing users to experience a stereo image with both a high depth of field and high resolution. With FusionOptics, the right channel delivers an image with the highest possible resolution, while the left channel provides an image with maximum depth of field. The human brain does the rest. It creates a 3-dimensional image providing the user with an image that has high depth of field and high resolution. Typical stereo microscope systems are designed to push magnification, resolution, and image quality to the limit permitted by optics. These limits are determined by the correlation between resolution, convergence angle, and working distance. Working beyond these limits presents users with imaging problems like empty magnification, exaggerated depth of field, and shorter working distances. The higher the microscope resolution, the higher the convergence angle between the left and right beam paths. This results in lowering the available working distance to the user potentially compromising sample manipulation. However, increasing the distance between the optical axes would cause the 3-dimensional image seen by the observer to become distorted. A greater zoom range alone is of little use, since with increasing magnification there is not an increase in optical resolution. The result is what is known as empty magnification. FusionOptics technology found in many Leica Microsystems stereo microscopes, such as the new M205 FCA, breaks these optical limitations by using two beam paths for different tasks. The right channel delivers a high resolution image at the largest possible numerical aperture, while the left channel presents an image with the high depth of field. The two images are merged in the human brain providing the user with an image with outstanding richness of detail and outstanding depth of field at the same time. This allows you to see more of your sample and provides the necessary working distance to comfortably manipulate the sample. The Leica M205 FCA with FusionOptics provides researchers with the resolution and working distance required by their application.
Views: 882 LeicaMicrosystems
Wi-Fi Education Solutions by Leica Microsystems: Letting minds meet … anywhere
 
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Discover a new dynamic in science class! The stereo microscope Leica EZ4 W with integrated Wi-Fi camera and the wireless Leica ICC50 W camera for compound microscopes stream HD images directly to mobile devices via AirLab App. Learning becomes more interactive and exciting. Microscope Camera Leica ICC50 W: http://www.leica-microsystems.com/products/microscope-cameras/education/details/product/leica-icc50-w/ Stereo Microscope Leica EZ4 W: http://www.leica-microsystems.com/products/stereo-microscopes-macroscopes/details/product/leica-ez4-w/
Views: 2025 LeicaMicrosystems
Introducing the Leica LAS User Interface
 
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This video shows a brief overview on the main navigation steps of the Leica LAS workflow. Be impressed by how easy it is to SETUP, AQUIRE, BROWSE, PROCESS and ANALYSE images.
Views: 5531 LeicaMicrosystems
Cataract Surgery, Case 1, Leica M822 Ophthalmic Microscope
 
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This live surgery video shows the ultimate Red Reflex in cataract surgery with the Leica M822 surgical microscope. Please visit http://www.leica-microsystems.com
Views: 8941 LeicaMicrosystems
GLOW800 Augmented Reality (AR) Fluorescence Imaging
 
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GLOW800 AR fluorescence is a new approach to vascular fluorescence during neurosurgery from Leica Microsystems. It combines the high contrast of near infrared (NIR) fluorescence imaging with the full visual spectrum of white light into a single, real-time image. By overlaying the colored AR fluorescence image in the oculars, the surgeon can see the white light image combined with colored NIR fluorescence flow in real-time, providing a more complete view of anatomical structures with no interruption to workflow. For more information on GLOW800 AR fluorescence, please visit: https://www.leica-microsystems.com/products/surgical-microscopes/details/product/glow800/
Views: 5193 LeicaMicrosystems
Toric IOL positioning with IOLcompass Pro and the Proveo 8 microscope
 
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This video from Dr. James Katz, The Midwest Center for Sight, Des Plaines, Illinois shows how IOLcompass Pro guidance software integrated into the Proveo 8 ophthalmic microscope can support cataract surgery. Compare positioning accuracy with IOLcompass Pro to blue ink marking. See how IOLcompass Pro accurately aligns templates to the limbus and maintains template stability throughout the procedure thanks to its sophisticated algorithms.
Views: 1347 LeicaMicrosystems
Eurospine 2018: Leica Microsystems launches the new PROVIDO surgical microscope
 
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Check out the first exciting impressions about the new PROVIDO spine microscope launched at Eurospine in Barcelona on September 19. You can still meet us at the Leica Booth #38B until Friday to discover the brand-new microscope dedicated to Microsurgery, PROVIDO to see more, simply. Want to see more, simply for your spine surgeries? Visit us: https://bit.ly/2DdWTkR
Views: 1913 LeicaMicrosystems
Leica Microsystems' innovations in surgical microscopy featured on renowned television series
 
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Leica Microsystems’ Medical Division recently was featured during the nationally televised program "Innovations with Ed Begley Jr." (http://innovationstelevision.com/). Four prominent surgeons and Leica Microsystems President Markus Lusser discussed the many ways Leica Microsystems is advancing surgical microscopy by integrating enhanced digital technologies directly into the operating microscope. Watch the full segment above. Press release: http://www.prweb.com/releases/2017/08/prweb14578542.htm
Views: 2057 LeicaMicrosystems
The Leica DMi8: Modularity
 
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As your research and lab grows, the Leica DMi8 is able to grow with you. Its unique flexible design allows you to configure and build a microscope that fits your needs and budget today, while offering upgradability for future research needs.
Views: 2575 LeicaMicrosystems
Leica EM AMW Automatic Microwave Tissue Processor for Electron Microscopy
 
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Leica EM AMW - Automatic Microwave Tissue Processor for Electron Microscopy. With the Leica EM AMW, tissue can be processed, embedded and polymerised into resin for subsequent analysis with unmatched automation, in hours rather than days. Automated microwave tissue processing for routine, life science and medical research EM applications.
Views: 4129 LeicaMicrosystems
Vessel suture with the Proveo 8 Ophthalmic Microscope | André Maia, MD
 
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Recording of a vessel suture by André Maia, MD, Retina Clinic, São Paulo, Brasil using a Proveo 8 ophthalmic microscope. The video demonstrates the visualization capabilities of FusionOptics, an exclusive optical approach from Leica that unites high resolution and depth of field for a texture-rich view of fine details from the vitreous to the retina without frequent refocusing. Find out more about the Proveo 8 premium ophthalmic microscope: http://www.leica-microsystems.com/products/surgical-microscopes/ophthalmology/details/product/leica-proveo-8/ Video provided courtesy of André Maia, MD.
Views: 1680 LeicaMicrosystems
Combining LightSheet and Confocal – The Vertical Turn: Leica TCS SP8 DLS
 
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Does your heart beat for light sheet microscopy too? With the new Leica TCS SP8 DLS, Leica Microsystems makes a turn in light sheet microscopy. The new light sheet module is fully implemented into the Leica TCS SP8 confocal platform. Learn more about light sheet imaging by Leica Microsystems: http://www.leica-microsystems.com/products/confocal-microscopes/details/product/leica-tcs-sp8-dls/ Sample: Beating heart of zebrafish embryo, transgenic line flik1::EGFP. Movie Stack recording, post-acquisition alignment. Courtesy of Emily Steed, Vermot Lab, IGBMC Strasbourg, France.
Views: 6751 LeicaMicrosystems
From Sample Overview to High Res Scans with LAS X Navigator
 
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Save yourself time when making high resolution scans of specific regions of interest on your sample/specimen using the Leica Application Suite X (LAS X) Navigator software. This video demonstrates how you can use previously recorded overview images of the entire specimen to acquire fast high resolution scans of interesting regions. Whether simple or advanced life science research tasks, see more with the LAS X Navigator platform. Learn more about our software platform LAS X for Life Science: https://www.leica-microsystems.com/products/microscope-software/details/product/leica-las-x-ls/ Final Video Transcript Video Title: LAS X Navigator Software: From Quick Sample Overview to Fast High Resolution Scans Text: There are many ways to find regions of interest on your sample. One way is to generate an overview image  for example, using the spiral scan mode. However, the fastest way is to use an already acquired overview image. In the example shown here, the overview image was taken with a mobile phone camera. The first step is to take some images of the sample with the microscope, like these here. Then load the overview image from the phone. Now align the sample and overview images by defining corresponding reference points on each. Press “Align” and then check alignment by looking at the overlay image. It looks good. And now the mobile phone image can be used for navigation. Now define, for example, two regions for a high resolution scan using the Magic Wand tool. Change to the desired magnification. And start the scan. The results of the stitched images are seen under Mosaic Merge. Here the raw data and here the merged data. Same with the second region: raw data and merged data. Regions can also be merged while keeping a spatial relationship corresponding to that of the sample. A flawless, continuous image is produced after stitching of the tile images. The data can be used for any further processing or analysis steps. Go quickly from sample overview to high resolution scans with the LAS X Navigator software.
Views: 1624 LeicaMicrosystems
Modern Cleanliness Analysis for Your Quality Assurance by Leica Microsystems
 
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Leica Microsystems' Cleanliness Expert solutions enhance workflow productivity through intuitive software and generate reports in compliance with user-specifications or common standards such as ISO 16232 or VDA 19. Read more: http://www.leica-microsystems.com/products/microscope-software/materials-sciences/details/product/leica-cleanliness-expert/
Views: 5370 LeicaMicrosystems
Leica EM TIC 3X  -  Triple Ion-Beam Cutter
 
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The technology of the Leica EM TIC 3X surpasses conventional slope cutting instruments. With its three ion beams, cooling stage and multiple sample stage the Leica EM TIC 3X can mill at high rates, cut broad and deep into the sample and create smooth surfaces resulting in quality cross-sections for almost any material, quickly and easily.
Views: 8787 LeicaMicrosystems
Leica EM SPF - Self Pressure Freezer
 
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The Leica EM SPF Self Pressure Freezer is a system suited for: - Cryo-immobilization of biological samples - Follow-on procedures for cryo- and room temperature TEM
Views: 1362 LeicaMicrosystems
Improve 3D Cell Biology with Digital LightSheet Microscopy from Leica Microsystems
 
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A constantly ascending number of culturing methods allows cancer cells to be grown as 3D spheres. The complex 3D structure and volumetric size represents a challenge in conventional light microscopy. This can be overcome by light sheet-based microscopy: http://www.leica-microsystems.com/applications/life-science/3d-biology-workflow/
Views: 1342 LeicaMicrosystems
Leica RUV800 Tradeshow video - panoramic retinal view system
 
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This Leica RUV800 product Tradeshow video is specifically designed to show the outstanding panoramic retinal view system in combination with the Leica M822 surgical microscope. Please visit http://www.leica-microsystems.com
Views: 2665 LeicaMicrosystems
Image samples from mini to massive with the DVM6 M digital microscope
 
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DVM6 M digital microscopes are ideal for inspection of automotive and aerospace parts, fabrications and assemblies, castings, bearings, valves, seals, welds and much more. Inspect samples as tall as 14.5" (37cm) with the clarity and resolution to capture the details you need to drive quality.
Views: 1763 LeicaMicrosystems
High Speed Scanning - Leica TCS SP8 with Tandem Scanner
 
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High speed scanning is necessary to image rapidly changing biological processes. With traditional scanning techniques, imaging speed is limited by the number of fluorophores in a specimen. And, rapid acquisition often comes at the cost of image resolution. Leica Microsystems’ Tandem Scanner eliminates the need for compromise by providing two scanners in one system. This allows you to optimize acquisition parameters based on your experimental needs. The Leica TCS SP8 combines the FOV scanner, for the widest field of view and image format, with a second high speed resonant scanner. The Leica TCS SP8 with tandem scanner allows you to choose between a large field of view and high speed for uncompromised imaging that adapts to every experiment. Learn more at: http://www.leica-microsystems.com/products/confocal-microscopes/leica-tcs-sp8-configurable-confocal/technology/tandem-scanner/
Views: 1927 LeicaMicrosystems
Leica M822 Tradeshow video -  Red reflex in cataract surgery
 
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This Leica M822 product Tradeshow video is specifically designed to show the ultimate Red Reflex in cataract surgery with the Leica M822 surgical microscope. Please visit http://www.leica-microsystems.com
Views: 3645 LeicaMicrosystems
Fast, Precise Serial Sectioning for Array Tomography: the new ARTOS 3D Ultramicrotome
 
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Now you can make high quality, aligned, serial sections for great array tomography results in half the time! Just use the ARTOS 3D ultramicrotome from Leica Microsystems. It expands 3D image reconstruction applications for cell biology research. The ARTOS 3D is a powerful ARray TOmography solution. It offers a new level of quality and speed for ultramicrotomy in terms of sectioning for applications like array tomography, using fluorescence or electron microscopy, and correlative light and electron microscopy (CLEM). More details are available here: https://bit.ly/2IKzVBb . Video Script Uniform, aligned, ultrathin serial sections in 50% less time. Excellent array tomography (AT) results. The ARTOS 3D sectioning solution for array tomography (AT). Fast carrier transfer with high section density for a seamless AT workflow. No section ribbon wrinkling: adjustable water flow Automated serial sectioning producing uniform ribbons. 40 nm thick sections. 50 sections per ribbon. Seamless auto-transition. Thicker sections for a smooth “knife edge release” of the AT ribbons. Collect ribbons wrinkle-free with ease. 150 sections on a 25 x 25 mm Si wafer. Quickly remove the section carrier with aligned ribbons. Easily sputter coat it and load it in the SEM. Array tomography results, Mouse lymph node (Courtesy IST Austria) 3D view of the paracortex of a popliteal lymph node. Acquired with SEM micrograph stack. For 3D image reconstruction, SEM image data set aligned. 3D reconstruction of T-cells in lymph nodes showing sub-cellular structures. T-cell cytoplasm (green), nuclei (blue/violet), and mitochondria (yellowish brown).
Views: 463 LeicaMicrosystems
Leica EM ACE200
 
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The Leica EM ACE200 is a fully automated, high quality desk-top coater designed to produce homogenous coatings of conductive metal or carbon. This is a pre-requisite for EM (Electron Microscopy). The instrument can be configured as a sputter coater, carbon thread evaporation coater and a version combining both methods. Read more: http://www.leica-microsystems.com/products/em-sample-preparation/biological-specimens/room-temperature-techniques/coating/
Views: 2555 LeicaMicrosystems
How to do a proper cell culture quick check
 
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Many fields of biomedical research, like cancer research, drug development and tissue engineering, require the use of living cells to perform a variety of assays. Mammalian cell cultures are an essential tool in biology because they allow rapid growth and proliferation of different cell types for experimental analysis. In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth must be monitored at regular intervals. When a cell line reaches about 80% confluence, the cells must be subcultured to ensure proper growth and health of the cells. 80% confluency means when 80% of the surface of a culture vessel is covered with cells. This video shows a typical workflow for subculturing an adherent cell line with detailed illustrations of all of the necessary steps. More information about the Leica DMi1, the microscope used in the video: http://www.leica-microsystems.com/products/light-microscopes/life-science-research/inverted-microscopes/details/product/leica-dmi1/
Views: 3182 LeicaMicrosystems
Leica EM ACE600
 
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The Leica EM ACE600 is a fully automated table-top coater, designed to produce very thin, fine-grained and conductive metal and carbon coatings for the highest resolution analysis, as required for FE-SEM and TEM applications. Read more: http://www.leica-microsystems.com/products/em-sample-preparation/biological-specimens/room-temperature-techniques/coating/
Views: 3495 LeicaMicrosystems
Leica EM GP Automatic Plunge Freezer for the Bare Grid Technique
 
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Leica EM GP - Automatic Plunge Freezer for the Bare Grid Technique. Leica Microsystems has developed a plunge freezer, in conjunction with Dr. Guenter REsch of IMP/IMBA Electron Microscopy Facility in Vienna, Austria, to standardize procedures and make the bare grid technique more reproducible
Views: 4579 LeicaMicrosystems
Neurobiology: Study synaptic dynamics with millisecond precision
 
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Recycling of synaptic vesicles is an extremely fast process. What if you could dissect the synaptic dynamics with millisecond precision? To resolve controversial questions in neurobiology employing new techniques for visualizing cellular activity is essential. The Leica high pressure freezer, EM ICE, with electrical stimulation offers millisecond precision to study neural circuits. High-pressure freezing allows for the near-instantaneous immobilization of cells. By varying the time intervals between stimulation and freezing, membrane trafficking within synaptic terminals can be captured following the induction of an action potential. The samples investigated are time slices that allow to dissect such highly dynamic processes. For more information visit http://www.leica-microsystems.com/synapticdynamics Workflow with the EM ICE: - Insert sample on PCB – Electrical stimulation of the sample (freely programmable) – High pressure freezing Synchronize freezing and stimulation to the millisecond to capture the moment that interests you most. EM ICE is giving neurobiology a tool to dissect fast action potential and membrane trafficking events. EM ICE - Capture the moment of your discovery!
Views: 782 LeicaMicrosystems
Intraventricular Brain Tumor Resection | Vik Udani, MD
 
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Recording of an intraventricular brain tumor resection by Vik Udani, MD, Division of Neurosurgery, Senta Clinic, San Diego, CA. A 79-year-old man presented with altered mental status and frequent falls. He was noted to have large mass with obstructive hydrocephalus. Case and video provided courtesy of Vik Udani, MD.
Views: 4117 LeicaMicrosystems
Progress Seamlessly during your ENT surgeries with PROVIDO
 
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Hear what clinical experts say about the new PROVIDO ENT microscope. It was developed, using the unique FusionOptics technology, to meet your challenges in ENT surgery. For more information about PROVIDO or to make a demo request: https://www.leica-microsystems.com/products/surgical-microscopes/details/product/provido-1/
Views: 759 LeicaMicrosystems
Leica EM ACE600 table-top coater with Leica VCT100
 
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The Leica EM ACE600 is a fully automated table-top coater, designed to produce very thin, fine-grained and conductive metal and carbon coatings for the highest resolution analysis, as required for FE-SEM and TEM applications. Outfitted with the Leica EM VCT100 (vacuum cryo transfer system) this instrument is the ideal solution for contamination-free cryo-SEM sample preparation with complete environmental control. Read more: http://www.leica-microsystems.com/products/em-sample-preparation/biological-specimens/room-temperature-techniques/coating/
Views: 2348 LeicaMicrosystems
World debut of the ARveo Digital Augmented Reality microscope for neurosurgery at AANS 2018
 
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Take a look at the world debut of the ARveo digital augmented reality microscope at AANS in New Orleans, USA. Find out what leading surgeons think about the benefits of integrated technologies, such as GLOW800 augmented reality vascular fluorescence, 3D visualization, and IGS integration. Watch interviews with Maxim Mamin, Vice President Medical Division, Leica Microsystems, Dr. Gustavo Pradilla, Assistant Professor, Emory University Dept. of Neurosurgery USA, and Dr. Justin Singer, Director of Vascular Neurosurgery, Spectrum Health, USA. The ARveo digital augmented reality microscope with groundbreaking GLOW AR fluorescence technology augments the intra-operative view and empowers decision-making with a single, complete picture of what lies ahead. ARveo includes: - Augmented digital imaging for precise, real-time information - The freedom to choose what you see and how you see it - Flexible options to share the surgical view in the OR and beyond Find out more: https://www.leica-microsystems.com/products/surgical-microscopes/details/product/arveo/
Views: 771 LeicaMicrosystems
90% time savings for microstructure composition analysis
 
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Image the microstructure and analyze its material composition in a single work step. You now have a 2-in-1 solution (visual and chemical analysis) - LIBS (laser induced breakdown spectroscopy) combined with the DM6 M microscope. More information: https://www.leica-microsystems.com/products/light-microscopes/upright-microscopes/details/product/libs-module/ Transcript Hi Guys, I’m Kenny Perry. I work for Leica Microsystems. I would like to briefly run through a quick 10,000-foot (bird’s-eye) view of our new LIBS device which is a Laser Induced Breakdown Spectroscopy tool. It is a laser attachment for our DM6 M fully automated optical microscope. It is very simple to use. The primary purpose of this tool is to give you an optical image and also elemental analysis with no sample preparation and without the use of a SEM (scanning electron microscope). So I will run through a very quick demo. You can see our workflow is very simple. You simply press "prepare measurement" and then "start measurement". At this point, we are going to use a nitrogen laser to blast our sample and penetrate about 5 microns (µm). And then our results will pop up within 3 seconds. You can see in this particular case, our sample is aluminum (Al) with traces of chromium (Cr), titanium (Ti), and platinum (Pt). So the takeaway is that this is an extremely easy-to-use tool and it does 2 things for you. It gives you an (1) optical image and (2) elemental analysis in 1 cost-effective tool. For more information, you can check out our website or look on our YouTube page.
Views: 836 LeicaMicrosystems
Save up to 20% inspection and rework time with the S9 stereo microscopes
 
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Save up to 20% inspection and rework time with the S9 stereo microscopes The S9 stereo microscopes help you to optimize your production workflow, keep defect rates low, and fulfill customer requests effortlessly. You can find details quickly and work up to 20% faster in inspection and rework. For more information, go to https://www.leica-microsystems.com/products/stereo-microscopes-macroscopes/routine-manual/details/product/leica-s9-e/ ************************** Video transcript: Optimize your workflow! Save up to 20% inspection and rework time* *compared to the standard industrial stereo microscope S6 from Leica Microsystems The Leica S9 Greenough Stereo Microscopes help you to speed up your optical inspections Operators become faster as they spend less time adjusting the microscope Fast from overview to detail: 9:1 zoom & up to 55x magnification Fast orientation in 3D: Large field of view & high depth of field Fast detection of details: FusionOptics for large depth of field and high resolution at the same time Fast sharing of results: S9 i with integrated 10 MP camera and network connectivity Save time with the S9 series from Leica Microsystems
Views: 455 LeicaMicrosystems
Photomanipulation with DMi8 S: Flexible live cell imaging
 
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The Leica DMi8 S microscopy system lets you easily combine applications by adding 1 or more advanced fluorescence imaging modules, such as the Infinity Scanner and Infinity TIRF. Get unprecedented flexibility allowing you to expose your sample to multiple techniques simultaneously, giving you deeper insights into your cells. The Infinity Scanner enables a host of photomanipulation techniques, like FRAP, FLIP, photo activation as well as photo switching, optogenetics and ablation. Advance your research by combining several photo manipulation modes in one experiment, e.g. apply photo damage to the nucleus to induce DNA damage. Then use photo switching to identify and track when repair proteins are released from the DNA. Setting up and running experiments is straightforward, allowing you to see the hidden to advance your discoveries. The Leica DMi8 S offers near infinite possibilities to adapt and expand with your research and enable your discoveries today and tomorrow. Go to the DMi8 S website: https://www.leica-microsystems.com/products/light-microscopes/inverted-microscopes/details/product/leica-dmi8/
Views: 880 LeicaMicrosystems

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